The floor and the walls of the restroom were contaminated after toilet flushing, but no significant differences were observed between the contamination occurring with lid position up or down. Wall contamination was minimal, regardless of lid position, and there was no significant difference in contamination level between the surfaces assessed, but data indicated that the trajectory of the aerosol plume contamination may have changed. Floor contamination was not found to be reduced consistently by toilet lid closure prior to flushing. […] it appears that closure of the toilet lid is not effective in reducing the contamination of the toilet seat area or contamination of other areas (fomites, floors, walls) within the restroom.